Lyophilized from 0.22 µm filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE
Form:
Lyophilized
Sequence:
Ser320-Arg429
Target:
GDF-2/BMP-9
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 0.4-4 ng/mL (QC Test).
Human GDF-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Immobilized Human GDF-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF-2 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
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