Human GDF15 Protein, Ultra Low Endotoxin (GDF-HM215-UL)

Product Overview

• Article Name: Human GDF15 Protein, Ultra Low Endotoxin (GDF-HM215-UL)
• Biozol Catalog Number: KAB-GDF-HM215-UL
• Supplier Catalog Number: GDF-HM215-UL
• Alternative Catalog Number: KAB-GDF-HM215-UL-100UG, KAB-GDF-HM215-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: GDF-15, MIC-1, NAG-1, PDF, PLAB, PTGFB, GDF15, MIC1, RG-1, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta

Product Description

Recombinant Human GDF15 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala197-Ile308.

Product Properties

• Molecular Weight: The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Tag: N-hFc (IgG1)
• UniProt: Q99988
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala197-Ile308
• Target: GDF15

Images

Human GDF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GDF15 is greater than 95% as determined by SEC-HPLC.

Immobilized Human GFRAL, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA (QC Test).

Loaded Human GDF15, hFc Tag on ProA-Biosensor can bind Human GFRAL, His-Avi Tag with an affinity constant of 35.50 nM as determined in BLI assay (Gator Prime).

Immobilized Human GDF15, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-GDF15 Antibody, hFc Avi Tag with the EC50 of 22.5ng/ml determined by ELISA.