Human GFRA3 Protein (GFR-HM1A3)

Product Overview

• Article Name: Human GFRA3 Protein (GFR-HM1A3)
• Biozol Catalog Number: KAB-GFR-HM1A3
• Supplier Catalog Number: GFR-HM1A3
• Alternative Catalog Number: KAB-GFR-HM1A3-100UG, KAB-GFR-HM1A3-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: GDNFR-alpha-3, GFR-alpha-3, GFRA3, GFR alpha3, GPI-linked receptor

Product Description

Recombinant Human GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp32-Trp382.

Product Properties

• Molecular Weight: The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: O60609
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asp32-Trp382
• Target: GFRA3

Images

Human GFRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GFRA3 is greater than 95% as determined by SEC-HPLC.

Immobilized Human GFRA3, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.

Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test).

Loaded Human GFRA3, His Tag on Anti-His-Biosensor can bind Human ARTN, hFc Tag with an affinity constant of 5.95 nM as determined in BLI assay (Gator Prime).