Lyophilized from 0.22 µm filtered solution in 20 mM Tris, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
E.coli
Purity:
95% as determined by SEC-HPLC.
Form:
Lyophilized
Sequence:
Asn49-Ser162
Target:
IL-15
Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 * 10 7-IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test).
GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80C and thawed at 37C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay.
GMP Human IL-15 on Bis-Tris PAGE under reduced condition.
GMP Human IL-15 (reconstituted protein) was stored at 4C, the protein is stable for 90 days determined by cell based assay.
GMP Human IL-15 (reconstituted protein) was stored at 37C, the protein is stable for 7 days determined by cell based assay.
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