T7 RNA Polymerase, GMP-Grade (GMP-T7P-EE101), DMF 037660 - Preis auf Anfrage

Product Overview

• Article Name: T7 RNA Polymerase, GMP-Grade (GMP-T7P-EE101), DMF 037660 - Preis auf Anfrage
• Biozol Catalog Number: KAB-GMP-T7P-EE101
• Supplier Catalog Number: GMP-T7P-EE101
• Alternative Catalog Number: KAB-GMP-T7P-EE101-1MU
• Manufacturer: KactusBio
• Category: Molekularbiologie

Product Description

T7 RNA Polymerase (T7RNAP) is the key enzyme for in vitro transcription (IVT) of mRNA. T7 Polymerase is a DNA-dependent polymerase that efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts. T7RNAP initiates transcription at the G* in the classic T7 promoter sequence-taatacgactcactataG*GG. T7 Polymerase is highly specific to its promoter sequence. When using the GAG cap1-Analog for co-transcription, taatacgactcactataA*GG is recommended. Note: GMP T7 RNA Polymerase requires the cofactor Mg2+ for mRNA synthesis. We recommend restriction enzymes that produce blunt ends or 5-overhangs. Weve filed our GMP T7RNAP with the FDA Drug Master Files (DMF) and its been assigned DMF 037660.

Product Properties

• Buffer: Liquid

Images

Figure 1. Three plasmids (lane 1 with poly A tail, lane 2 and 3 without poly A tail) were used as templates, and the reaction was performed for 2 hours at 37C in a 20µL system. The transcription length was 2000 nt, 4000 nt, and 2000 nt, respectively. The above mRNA was not purified.

Figure 2. Detection of T7 RNA Polymerase by molecular beacon. When the molecular beacon and transcript specifically bind, the conformation changes and the fluorescence intensity changes. Three batches of T7 RNA Polymerase were detected, and the slope was similar across all batches, indicating stable activity across batches.

Figure 3. Yield was assessed via nanodrop and Agilent 4150, and purity was assessed by CE. Overall, yield and purity of KACTUS T7 was comparable to leading competitors.