Human GM-CSF Protein (GSF-HE001)

Product Overview

• Article Name: Human GM-CSF Protein (GSF-HE001)
• Biozol Catalog Number: KAB-GSF-HE001
• Supplier Catalog Number: GSF-HE001
• Alternative Catalog Number: KAB-GSF-HE001-100UG, KAB-GSF-HE001-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CSF, CSF2, GMCSF, GM-CSF, MGC131935, MGC138897

Product Description

Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.

Product Properties

• Molecular Weight: The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P04141
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala18-Glu144
• Target: GM-CSF

Images

Human GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.

Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).

Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).