Human GITR/TNFRSF18 Protein (GTR-HM101)

Product Overview

• Article Name: Human GITR/TNFRSF18 Protein (GTR-HM101)
• Biozol Catalog Number: KAB-GTR-HM101
• Supplier Catalog Number: GTR-HM101
• Alternative Catalog Number: KAB-GTR-HM101-100UG, KAB-GTR-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD357, GITR, GITR-D, TNFRSF18, AITR

Product Description

Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Glu161.

Product Properties

• Molecular Weight: The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q9Y5U5
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln26-Glu161
• Target: GITR/TNFRSF18

Images

The purity of Human GITR is greater than 95% as determined by SEC-HPLC.

Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%

Immobilized Human GITR, His Tag at 0.5ug (100ul/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.

Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).

Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).