Human ICOS/CD278 Protein (ICO-HM201)

Product Overview

• Article Name: Human ICOS/CD278 Protein (ICO-HM201)
• Biozol Catalog Number: KAB-ICO-HM201
• Supplier Catalog Number: ICO-HM201
• Alternative Catalog Number: KAB-ICO-HM201-100UG, KAB-ICO-HM201-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: AILIM, CD278, CRP-1, CVID1, ICOS, MGC39850

Product Description

Recombinant Human ICOS/CD278 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu21-Phe141.

Product Properties

• Molecular Weight: The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q9Y6W8
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Glu21-Phe141
• Target: ICOS/CD278

Images

Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).

Immobilized Human B7-H2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).

Immobilized Human ICOS, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ICOS Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.

The purity of Human ICOS is greater than 90% as determined by SEC-HPLC.

Human ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.