Biotinylated Human IFN alpha/beta R2 Protein (IFN-HM140RB)

Product Overview

• Article Name: Biotinylated Human IFN alpha/beta R2 Protein (IFN-HM140RB)
• Biozol Catalog Number: KAB-IFN-HM140RB
• Supplier Catalog Number: IFN-HM140RB
• Alternative Catalog Number: KAB-IFN-HM140RB-100UG, KAB-IFN-HM140RB-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: IFN-R-2, IFNAR2, IFNARB, IFNABR, IFN-alpha, IFN-alpha/beta R2, IFNbR2, IFN-R, beta receptor 2, beta receptor10,

Product Description

Recombinant Biotinylated Human IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile27-Lys243.

Product Properties

• Molecular Weight: The protein has a predicted MW of 27.66 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P48551
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ile27-Lys243
• Target: IFN alpha/beta R2

Images

The purity of Biotinylated Human IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 24.2 nM as determined in SPR assay (Biacore T200).

Loaded Biotinylated Human IFN alpha/beta R2, His-Avi Tag on Anti-His-Biosensor can bind Human IFN alpha 1, hFc Tag with an affinity constant of 13.20 nM as determined in BLI assay (Gator Prime).

Biotinylated Human IFN alpha/beta R2,His-Avi Tag captured on CM5 Chip via Streptavidin can bind Human IFN alpha 1, hFc Tag with an affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).