Human IFN alpha 1 Protein (IFN-HM2A1)

Product Overview

• Article Name: Human IFN alpha 1 Protein (IFN-HM2A1)
• Biozol Catalog Number: KAB-IFN-HM2A1
• Supplier Catalog Number: IFN-HM2A1
• Alternative Catalog Number: KAB-IFN-HM2A1-100UG, KAB-IFN-HM2A1-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: Interferon alpha-1/13,IFN-alpha-1/13,Interferon alpha-D,LeIF D, IFNalpha 1, IFN alpha1,IFNA1,IFN-alpha-1,Interferon alpha 1

Product Description

Recombinant Human IFN alpha 1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Cys24-Glu189.

Product Properties

• Molecular Weight: The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: P01562
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Cys24-Glu189
• Target: IFN alpha 1

Images

Human IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.

Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200) (QC Test).

Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).

Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator).