Recombinant Human IL-13 Protein is expressed from E.coli without tag.It contains Gly21-Asn132.
Molecular Weight:
The protein has a predicted MW of 12.34 kDa same as Bis-Tris PAGE result.
Tag:
No Tag
UniProt:
AAK53823
Buffer:
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
E.coli
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly21-Asn132
Target:
IL-13
The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC.
Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 3.6ng/ml determined by ELISA.
Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50-for this effect is typically 0.5-3 ng/mL.
Loaded Human IL-13Ra2, His Tag on Anti-His-Biosensor can bind Human IL-13, No Tag with an affinity constant of 0.63 nM as determined in BLI assay (Gator Prime).
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