Human IL-10 Protein (IL1-HM010)

Product Overview

• Article Name: Human IL-10 Protein (IL1-HM010)
• Biozol Catalog Number: KAB-IL1-HM010
• Supplier Catalog Number: IL1-HM010
• Alternative Catalog Number: KAB-IL1-HM010-100UG, KAB-IL1-HM010-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: Interleukin-10, IL-10, CSIF, IL10A, TGIF, B-TCGF, GVHDS, MGC126450, MGC126451

Product Description

Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178.

Product Properties

• Molecular Weight: The protein has a predicted MW of 18.8 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P22301
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ser19-Asn178
• Target: IL-10

Images

Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC.

Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).

Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test).

Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test).