Recombinant Human IL-1R1 Protein is expressed from HEK293 without tag.It contains Leu18-Thr332.
Molecular Weight:
The protein has a predicted MW of 36.17 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Tag:
No Tag
UniProt:
NP_000868.1
Buffer:
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu18-Thr332
Target:
IL-1R1
The purity of Human IL-1R1 is greater than 95% as determined by SEC-HPLC.
Human IL-1R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Human IL-1 Beta, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-1R1, No Tag with an affinity constant of 11.37 nM as determined in SPR assay (Biacore T200) (QC Test).
Human IL-1R1, No Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His-Avi Tag with an affinity constant of 1.01 uM as determined in SPR assay (Biacore T200).
Loaded Human IL-1 Beta, His-Avi Tag on Anti-His-Biosensor, can bind Human IL-1R1, No Tag with an affinity constant of 9.42 nM as determined in BLI assay (Gator).
* VAT and and shipping costs not included. Errors and price changes excepted