Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu33-Trp320.
Molecular Weight:
The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu33-Trp320
Target:
IL-17R alpha/CD217
Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA.
Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA.
Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
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