Recombinant Cynomolgus IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-terminus of IL-23 alpha.It contains Val22-Pro189(IL-23 alpha) and Ile23-Ser328(IL-12 beta).
Molecular Weight:
The protein has a predicted MW of 19.52 kDa (IL-23 alpha) and 34.76 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 20-25 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Val22-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)
Target:
IL-23 alpha&IL-12 beta
The purity of Cynomolgus IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Cynomolgus IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
Human IL-23R, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 4.05 nM as determined in SPR assay (Biacore T200).
Loaded Human IL-23R, hFc Tag on ProA-Biosensor, can bind Cynomolgus IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.60 nM as determined in BLI assay (Gator).
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