Human IL-25/IL-17E Protein, Ultra Low Endotoxin (IL2-HM125-UL)

Product Overview

• Article Name: Human IL-25/IL-17E Protein, Ultra Low Endotoxin (IL2-HM125-UL)
• Biozol Catalog Number: KAB-IL2-HM125-UL
• Supplier Catalog Number: IL2-HM125-UL
• Alternative Catalog Number: KAB-IL2-HM125-UL-100UG, KAB-IL2-HM125-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL-25, IL-17E, IL17E, Interleukin-17E, interleukin-25

Product Description

Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.

Product Properties

• Molecular Weight: The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q9H293
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Tyr33-Gly177
• Target: IL-25/IL-17E

Images

Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.

Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 4.64ug/ml determined by ELISA (QC Test).

The purity of Human IL-25 is greater than 95% and the molecular weight of this protein is around 40-60 kDa as determined by SEC-MALS.