Biotinylated Mouse IL-22 Protein (IL2-ME422B)

Product Overview

• Article Name: Biotinylated Mouse IL-22 Protein (IL2-ME422B)
• Biozol Catalog Number: KAB-IL2-ME422B
• Supplier Catalog Number: IL2-ME422B
• Alternative Catalog Number: KAB-IL2-ME422B-100UG, KAB-IL2-ME422B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Mouse
• Conjugation: Biotin
• Alternative Names: Interleukin-22, IL-22, Cytokine Zcyto18, IL-TIF, IL-D110, TIFa, TIFIL-23, ZCYTO18

Product Description

Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.

Product Properties

• Molecular Weight: The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.
• Tag: N-His-Avi
• UniProt: Q9JJY9
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu34-Val179
• Target: IL-22

Images

Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.

Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).

Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator Prime).