Human IL-3 R alpha/CD123 Protein (IL3-HM1RA)

Product Overview

• Article Name: Human IL-3 R alpha/CD123 Protein (IL3-HM1RA)
• Biozol Catalog Number: KAB-IL3-HM1RA
• Supplier Catalog Number: IL3-HM1RA
• Alternative Catalog Number: KAB-IL3-HM1RA-100UG, KAB-IL3-HM1RA-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD123, IL-3R subunit alpha, IL3R, IL3RA, IL-3Ra, IL3RAY, IL3RX, IL3RY, MGC34174, IL-3 R alpha

Product Description

Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr19-Arg305.

Product Properties

• Molecular Weight: The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P26951
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Thr19-Arg305
• Target: IL-3 R alpha/CD123

Images

Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.

Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 uM as determined in SPR assay (Biacore T200).

Immobilized Human IL-3 R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).

Loaded Human IL-3, hFc Tag Tag on ProA-Biosensor, can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.55 µM as determined in BLI assay (Gator).