Human IL-4 Protein (IL4-HM001)

Product Overview

• Article Name: Human IL-4 Protein (IL4-HM001)
• Biozol Catalog Number: KAB-IL4-HM001
• Supplier Catalog Number: IL4-HM001
• Alternative Catalog Number: KAB-IL4-HM001-100UG, KAB-IL4-HM001-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL4, IL-4, interleukin 4, MGC79402, pitrakinra, B cell growth factor 1, BCDF, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, BSF-1

Product Description

Recombinant Human IL-4 Protein is expressed from HEK293 without tag.It contains His25-Ser153.

Product Properties

• Molecular Weight: The protein has a predicted MW of 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P05112
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: His25-Ser153
• Target: IL-4

Images

Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.

Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.4ng/ml determined by ELISA.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.35 ng/mL

Loaded Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) on ProA-Biosensor can bind Human IL-4, No Tag with an affinity constant of 0.54 nM as determined in BLI assay (Gator Prime).