Human IL-15 Protein (IL5-HE015)

Product Overview

• Article Name: Human IL-15 Protein (IL5-HE015)
• Biozol Catalog Number: KAB-IL5-HE015
• Supplier Catalog Number: IL5-HE015
• Alternative Catalog Number: KAB-IL5-HE015-100UG, KAB-IL5-HE015-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: L15, IL-15, IL-15MGC9721, Interleukin-15

Product Description

Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.

Product Properties

• Molecular Weight: The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P40933
• Buffer: Lyophilized from 0.22 µm filtered solution in 20 mM Tris, 150 mM NACI (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asn49-Ser162
• Target: IL-15

Images

Human IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.

Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).

Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 * 10 7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).

Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.