Human IL-7 Protein (IL7-HE001)

Product Overview

• Article Name: Human IL-7 Protein (IL7-HE001)
• Biozol Catalog Number: KAB-IL7-HE001
• Supplier Catalog Number: IL7-HE001
• Alternative Catalog Number: KAB-IL7-HE001-100UG, KAB-IL7-HE001-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL7, Interleukin-7, IL-7, Lymphopoietin-1, PBGF

Product Description

Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.

Product Properties

• Molecular Weight: The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P13232
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asp26-His177
• Target: IL-7

Images

Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200).

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50-for this effect is-0.100-1 ng/mL.

The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.

Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested).