Human IL-18BP Protein (IL8-HM1BP)

Product Overview

• Article Name: Human IL-18BP Protein (IL8-HM1BP)
• Biozol Catalog Number: KAB-IL8-HM1BP
• Supplier Catalog Number: IL8-HM1BP
• Alternative Catalog Number: KAB-IL8-HM1BP-100UG, KAB-IL8-HM1BP-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL-18BP, Igifbp

Product Description

Recombinant Human IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr31-Gly194.

Product Properties

• Molecular Weight: The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: O95998
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Thr31-Gly194
• Target: IL-18BP

Images

Human IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag (Cat. IL1-HE018) with an affinity constant of 0.41 nM as determined in SPR assay (Biacore T200).

Measured by its ability to inhibit the IL-18-induced response of human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.04 - 0.08 µg/mL in the presence of 30 ng/mL of recombinant Human IL-18.

The purity of Human IL-18BP is greater than 90% as determined by SEC-HPLC.

Loaded Human IL-18BP, His Tag on Anti-His-Biosensor can bind Human IL-18, No Tag (Cat. IL1-HE018) with an affinity constant of 1.80 nM as determined in BLI assay (Gator Prime).