Non-biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46R)

Product Overview

• Article Name: Non-biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46R)
• Biozol Catalog Number: KAB-ILR-HM46R
• Supplier Catalog Number: ILR-HM46R
• Alternative Catalog Number: KAB-ILR-HM46R-100UG, KAB-ILR-HM46R-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, IL6R, CD126, IL6RQ, gp80

Product Description

Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.

Product Properties

• Molecular Weight: The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P08887
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu20-Pro365
• Target: IL-6 R alpha/CD126

Images

Human IL-6 R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of- Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.

Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).

Loaded Human IL-6 R alpha, His Tag on Anti-His-Biosensor can bind Human IL-6, No Tag with an affinity constant of 30.80 nM as determined in BLI assay (Gator Prime).