Rat IL-13Ra2 Protein (ILR-RM2R2)

Product Overview

• Article Name: Rat IL-13Ra2 Protein (ILR-RM2R2)
• Biozol Catalog Number: KAB-ILR-RM2R2
• Supplier Catalog Number: ILR-RM2R2
• Alternative Catalog Number: KAB-ILR-RM2R2-100UG, KAB-ILR-RM2R2-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Rat
• Alternative Names: CD213a2, IL-13 R alpha 2, IL13BP, IL13R, IL13RA2, IL-13R subunit alpha-2, IL-13Ra2

Product Description

Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu24-Lys336.

Product Properties

• Molecular Weight: The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q8VHK6
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu24-Lys336
• Target: IL-13Ra2

Images

Rat IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC.

Rat IL-13Ra2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 93.96 pM as determined in SPR assay (Biacore T200).

Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test).

Loaded Rat IL-13Ra2, hFc Tag on ProA-Biosensor can bind Human IL-13, His Tag with an affinity constant of 2.53 nM as determined in BLI assay (Gator Prime).