Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29, with strong strand displacement activity and high processivity (up to more than 70 kbp). The enzyme has inherent 3-5 exonuclease activity and higher fidelity than most Taq enzymes. It is used for isothermal amplification in vitro.
Buffer:
Liquid
Phi29 DNA Polymerase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Rolling cycle amplification of pUC57 plasmid with phi29 DNA Polymerase, product was digested with BspQI and then analyzed with Agarose gel.
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