Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2)

Product Overview

• Article Name: Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2)
• Biozol Catalog Number: KAB-TNF-HM3R2
• Supplier Catalog Number: TNF-HM3R2
• Alternative Catalog Number: KAB-TNF-HM3R2-100UG, KAB-TNF-HM3R2-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD120b, Etanercept, p75TBPII, p75TNFR, TNF RII, TNF-R2, TNF-R75, TNFR80, TNFRSF1B, TNFR2, TBPII, TNFBR, TNFR1B

Product Description

Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu23-Asp257.

Product Properties

• Molecular Weight: The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Tag: C-mFc
• UniProt: P20333
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu23-Asp257
• Target: TNFR2/CD120b/TNFRSF1B

Images

Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).

Immobilized Human TNFR2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.

Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).

The purity of Human TNFR2 is greater than 90% as determined by SEC-HPLC.

Human TNFR2-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.