Non-biotinylated Human TNF alpha Protein (active trimer) (TNF-HM40A)

Product Overview

• Article Name: Non-biotinylated Human TNF alpha Protein (active trimer) (TNF-HM40A)
• Biozol Catalog Number: KAB-TNF-HM40A
• Supplier Catalog Number: TNF-HM40A
• Alternative Catalog Number: KAB-TNF-HM40A-100UG, KAB-TNF-HM40A-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: APC1 protein, Cachectin, DIF, TNF, TNFalpha, TNFATNF, TNFSF1A, TNFSF2, TNFA, TNFalpha, DIF, TNFSF2,TNF-alpha

Product Description

Recombinant Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val77-Leu233.

Product Properties

• Molecular Weight: The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P01375
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 90% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val77-Leu233
• Target: TNF alpha

Images

Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).

The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC.

Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).

The purity of Human TNF alpha is greater than 95% and the molecular weight of this protein is around 55-76 kDa as determined by SEC-MALS.