Human VEGF121 Protein, Ultra Low Endotoxin (VEG-HM021-UL)

Product Overview

• Article Name: Human VEGF121 Protein, Ultra Low Endotoxin (VEG-HM021-UL)
• Biozol Catalog Number: KAB-VEG-HM021-UL
• Supplier Catalog Number: VEG-HM021-UL
• Alternative Catalog Number: KAB-VEG-HM021-UL-100UG, KAB-VEG-HM021-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: VEGF, VEGFA, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609

Product Description

Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.

Product Properties

• Molecular Weight: The protein has a predicted MW of 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P15692
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala27-Arg147
• Target: VEGF121

Images

Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.

Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.

Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL.

Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.