Biotinylated Human VEGF R2/KDR Protein (VGF-HM4R2B)

Product Overview

• Article Name: Biotinylated Human VEGF R2/KDR Protein (VGF-HM4R2B)
• Biozol Catalog Number: KAB-VGF-HM4R2B
• Supplier Catalog Number: VGF-HM4R2B
• Alternative Catalog Number: KAB-VGF-HM4R2B-100UG, KAB-VGF-HM4R2B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: CD309, KDR, VEGFR, VEGFR2, VEGFR-21, FLK1, KRD1, Ly73

Product Description

Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Glu764.

Product Properties

• Molecular Weight: The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P35968
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala20-Glu764
• Target: VEGF R2/KDR

Images

Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His-Avi Tag with the EC50 of 1.27ug/ml determined by ELISA.

Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Biotinylated Human VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.

Serial dilutions of Anti-VEGF165 Antibody were added into Human VEGF165, No Tag: Biotinylated Human VEGF R2, His Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.30ug/ml.

The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.