IHC-plus(TM) VCL / Vinculin Antibody LS-B12145, Unconjugated, Polyclonal

Product Overview

• Article Name: IHC-plus(TM) VCL / Vinculin Antibody LS-B12145, Unconjugated, Polyclonal
• Biozol Catalog Number: LS-B12145-0.05
• Supplier Catalog Number: LS-B12145-0.05
• Alternative Catalog Number: LS-B12145-0.05
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A 23 AA peptide near the center of human Vinculin. The immunogen is located within aa 820-870 of Vinculin.
• Conjugation: Unconjugated
• Alternative Names: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Product Description

Vinculin antibody LS-B12145 is an unconjugated rabbit polyclonal antibody to Vinculin (VCL) from human. It is reactive with human, mouse and rat. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 7414
• Buffer: PBS containing 0.02% Sodium Azide
• Purity: Peptide Column Affinity Chromatography
• Form: PBS containing 0.02% Sodium Azide

Application Notes

• Application Dilute: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)
• Application Notes: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 ug/ml. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human sa

Images

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5 µg/ml.

Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20 µg/ml.