IHC-plus(TM) Polyclonal Rabbit anti-Human BUB3 Antibody (IHC, IF, WB) LS-B1340

Product Overview

• Article Name: IHC-plus(TM) Polyclonal Rabbit anti-Human BUB3 Antibody (IHC, IF, WB) LS-B1340
• Biozol Catalog Number: LS-B1340-0.05
• Supplier Catalog Number: LS-B1340-0.05
• Alternative Catalog Number: LS-B1340-0.05
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: A 16 AA synthetic peptide near the C-Terminus of human bub3. The immunogen is located within the last 50 AA of Bub3.
• Conjugation: Unconjugated
• Alternative Names: BUB3, Budding uninhibited by benomyl, HBUB3, BUB3L, Mitotic checkpoint component

Product Description

BUB3 antibody LS-B1340 is an unconjugated rabbit polyclonal antibody to BUB3 from human. It is reactive with human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 9184
• Buffer: PBS containing 0.02% Sodium Azide
• Purity: Peptide Column Affinity Chromatography
• Form: PBS containing 0.02% Sodium Azide

Application Notes

• Application Dilute: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)
• Application Notes: Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples, Immunoc

Images

Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 ug/ml.

Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 ug/ml.

Western blot of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 ug/ml.