IHC-plus(TM) MAP3K5 / ASK1 Antibody LS-B3205, Unconjugated, Polyclonal

Product Overview

• Article Name: IHC-plus(TM) MAP3K5 / ASK1 Antibody LS-B3205, Unconjugated, Polyclonal
• Biozol Catalog Number: LS-B3205-0.05
• Supplier Catalog Number: LS-B3205-0.05
• Alternative Catalog Number: LS-B3205-0.05
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: A 16 AA synthetic peptide near the C-Terminus of human ASK1. The immunogen is located within aa 1260-1310 of ASK1.
• Conjugation: Unconjugated
• Alternative Names: MAP3K5, ASK-1, ASK1, MEKK5, MEK kinase 5, MEKK 5, MAPK/ERK kinase kinase 5, MAPKKK5, MAP/ERK kinase kinase 5

Product Description

ASK1 antibody LS-B3205 is an unconjugated rabbit polyclonal antibody to ASK1 (MAP3K5) from human. It is reactive with human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 4217
• Buffer: PBS containing 0.02% Sodium Azide
• Purity: Peptide Column Affinity Chromatography
• Form: PBS containing 0.02% Sodium Azide

Application Notes

• Application Dilute: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB
• Application Notes: ASK1 antibody can be used for the detection of ASK1 by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples, Immu

Images

Immunofluorescence of ASK1 in 3T3 cells with ASK1 antibody at 20 ug/ml.

Western blot of ASK1 in SW1353 cell lysate with MAP3K5 / ASK1 Antibody at (A) 0.5, (B) 1, and (C) 2

Immunocytochemistry of ASK1 in A431 cells with MAP3K5 / ASK1 Antibody at 2 ug/ml.