IHC-plus(TM) Monoclonal Mouse anti-Human BUB1B / BubR1 Antibody (clone 2G5, IHC, WB) LS-B4289

Product Overview

• Article Name: IHC-plus(TM) Monoclonal Mouse anti-Human BUB1B / BubR1 Antibody (clone 2G5, IHC, WB) LS-B4289
• Biozol Catalog Number: LS-B4289-50
• Supplier Catalog Number: LS-B4289-50
• Alternative Catalog Number: LS-B4289-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC, IHC-P, PLA, RNA-I, WB
• Species Reactivity: Human
• Immunogen: BUB1B (AAH18739, 1 aa ~ 130 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L

Product Description

BubR1 antibody LS-B4289 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for ELISA, IHC, PLA, RNAi and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [2G5]
• NCBI: 701
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IHC, IHC-P (5 µg/ml), PLA, RNAi, WB
• Application Notes: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated). Proximity Ligation Assay (Cell).

Images

Western blot of BUB1B over-expressed 293 cel

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.