IHC-plus(TM) Monoclonal Mouse anti-Human p66 / SHC Antibody (clone 3F4, IHC, IF, WB) LS-B4416

Product Overview

• Article Name: IHC-plus(TM) Monoclonal Mouse anti-Human p66 / SHC Antibody (clone 3F4, IHC, IF, WB) LS-B4416
• Biozol Catalog Number: LS-B4416-50
• Supplier Catalog Number: LS-B4416-50
• Alternative Catalog Number: LS-B4416-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, PLA, WB
• Species Reactivity: Human
• Immunogen: SHC1 (AAH33925, 171 aa ~ 280 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: SHC1, p46 shc, p52 shc, SH2 domain protein C1, p66, SHCA, SHC, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A

Product Description

SHC antibody LS-B4416 is an unconjugated mouse monoclonal antibody to human SHC (p66). Validated for ELISA, IF, IHC, PLA and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.42 mg/ml
• Clone Designation: [3F4]
• NCBI: 6464
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (1 µg/ml), PLA, WB
• Application Notes: Western Blot (Cell lysate) - positive control A-431. Immunofluorescence (10 ug/ml) - positive control A-431 cells. Sandwich ELISA (Recombinant protein). In situ Proximity Ligation Assay (Cell).

Images

SHC1 monoclonal antibody clone 3F4 Western blot of SHC1 expression in A-431.

Immunofluorescence of monoclonal antibody to SHC1 on A-431 cells (antibody concentration 10 ug/ml).

Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml a