IHC-plus(TM) ARNT / HIF-1-Beta Antibody (clone 3D10) LS-B6007, Clone: [3D10], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) ARNT / HIF-1-Beta Antibody (clone 3D10) LS-B6007, Clone: [3D10], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6007-50
• Supplier Catalog Number: LS-B6007-50
• Alternative Catalog Number: LS-B6007-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: ARNT (AAH60838, 1 aa ~ 110 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: ARNT, ARNT protein, BHLHe2, HIF-1beta, HIF1-beta, HIF1BETA, TANGO, HIF-1-beta, HIF1B

Product Description

HIF-1-Beta antibody LS-B6007 is an unconjugated mouse monoclonal antibody to human HIF-1-Beta (ARNT). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [3D10]
• NCBI: 405
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Images

Western blot of ARNT expression in transfected 293T cell line.

Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged ARNT is approximately 0.03 ng/ml as a capture anti