IHC-plus(TM) EP300 / p300 Antibody (clone 1D2) LS-B6081, IgG1, Clone: [1D2], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) EP300 / p300 Antibody (clone 1D2) LS-B6081, IgG1, Clone: [1D2], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6081-50
• Supplier Catalog Number: LS-B6081-50
• Alternative Catalog Number: LS-B6081-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: EP300 (NP_001420, 731 aa ~ 831 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2

Product Description

P300 antibody LS-B6081 is an unconjugated mouse monoclonal antibody to human p300 (EP300). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.3 mg/ml
• Clone Designation: [1D2]
• Isotype: IgG1
• NCBI: 2033
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Images

Western blot of EP300 expression in HeLa nuclear extract.

Immunofluorescence of monoclonal antibody to EP300 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged EP300 is approximately 0.03 ng/ml as a capture antibod