IHC-plus(TM) NDN / Necdin Antibody (clone 1B3) LS-B6103, Clone: [1B3], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) NDN / Necdin Antibody (clone 1B3) LS-B6103, Clone: [1B3], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6103-50
• Supplier Catalog Number: LS-B6103-50
• Alternative Catalog Number: LS-B6103-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: NDN (NP_002478, 222 aa ~ 322 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: NDN, Necdin, Necdin homolog (mouse), HsT16328, Necdin (mouse) homolog, PWCR

Product Description

Necdin antibody LS-B6103 is an unconjugated mouse monoclonal antibody to human Necdin (NDN). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [1B3]
• NCBI: 4692
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of NDN expression in transfected 293T cell line by NDN monoclonal antibody.

Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged NDN is approximately 0.03