IHC-plus(TM) SP1 Antibody (clone 4C8) LS-B6149, IgG1, Clone: [4C8], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) SP1 Antibody (clone 4C8) LS-B6149, IgG1, Clone: [4C8], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6149-50
• Supplier Catalog Number: LS-B6149-50
• Alternative Catalog Number: LS-B6149-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: SP1 (NP_612482, 89 aa ~ 199 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: SP1, Sp1 transcription factor, Transcription factor Sp1, Specificity protein 1, TSFP1

Product Description

SP1 antibody LS-B6149 is an unconjugated mouse monoclonal antibody to human SP1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.27 mg/ml
• Clone Designation: [4C8]
• Isotype: IgG1
• NCBI: 6667
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (20 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of SP1 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml]

Detection limit for recombinant GST tagged SP1 is approximately 0.3 ng/ml as a capture antibody.