IHC-plus(TM) UBTF / UBF Antibody (clone 1A2) LS-B6166, Clone: [1A2], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) UBTF / UBF Antibody (clone 1A2) LS-B6166, Clone: [1A2], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6166-50
• Supplier Catalog Number: LS-B6166-50
• Alternative Catalog Number: LS-B6166-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human, Rat
• Immunogen: UBTF (NP_055048, 551 aa ~ 651 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: UBTF, Autoantigen NOR-90, UBF, NOR-90, UBF-1, UBF1, Upstream-binding factor 1

Product Description

UBF antibody LS-B6166 is an unconjugated mouse monoclonal antibody to UBF (UBTF) from human. It is reactive with human and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5 mg/ml
• Clone Designation: [1A2]
• NCBI: 7343
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of UBTF expression in PC-12.

Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged UBTF is approximately 1 ng/ml as a capture antibody.