IHC-plus(TM) SYMPK / Symplekin Antibody (clone 4C2) LS-B6171, Clone: [4C2], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) SYMPK / Symplekin Antibody (clone 4C2) LS-B6171, Clone: [4C2], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6171-50
• Supplier Catalog Number: LS-B6171-50
• Alternative Catalog Number: LS-B6171-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: SYMPK (AAH30214, 1 aa ~ 534 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: SYMPK, Symplekin, SYM, SPK

Product Description

Symplekin antibody LS-B6171 is an unconjugated mouse monoclonal antibody to human Symplekin (SYMPK). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5 mg/ml
• Clone Designation: [4C2]
• NCBI: 8189
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of SYMPK expression in COLO 320 HSR.

Immunofluorescence of monoclonal antibody to SYMPK on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged SYMPK is approximately 0.03 ng/ml as a capture antibody.