IHC-plus(TM) MED17 / TRAP80 Antibody (clone 4D4) LS-B6197, Clone: [4D4], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) MED17 / TRAP80 Antibody (clone 4D4) LS-B6197, Clone: [4D4], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6197-50
• Supplier Catalog Number: LS-B6197-50
• Alternative Catalog Number: LS-B6197-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: CRSP6 (AAH21101, 551 aa ~ 652 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: MED17, CRSP77, CRSP complex subunit 6, CRSP6, DRIP77, Mediator complex subunit 17, TRAP80, ARC77, DRIP80

Product Description

TRAP80 antibody LS-B6197 is an unconjugated mouse monoclonal antibody to human TRAP80 (MED17). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [4D4]
• NCBI: 9440
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (1.2 - 5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of CRSP6 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to CRSP6 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CRSP6 is approximately 0.3 ng/ml as a capture antibody.