IHC-plus(TM) SAE1 Antibody (clone 1G4-1G5) LS-B6203, Clone: [1G4-1G5], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) SAE1 Antibody (clone 1G4-1G5) LS-B6203, Clone: [1G4-1G5], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6203-50
• Supplier Catalog Number: LS-B6203-50
• Alternative Catalog Number: LS-B6203-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: SAE1 (AAH00344, 1 aa ~ 347 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: SAE1, Activator of SUMO1, AOS1, UBLE1A, HSPC140, SUA1

Product Description

SAE1 antibody LS-B6203 is an unconjugated mouse monoclonal antibody to human SAE1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [1G4-1G5]
• NCBI: 10055
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (3 - 5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of SAE1 expression in HeLa.

Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged SAE1 is approximately 0.1 ng/ml as a capture antibody.