IHC-plus(TM) CHEK2 / CHK2 Antibody (clone 4B7) LS-B6212, Clone: [4B7], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) CHEK2 / CHK2 Antibody (clone 4B7) LS-B6212, Clone: [4B7], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6212-50
• Supplier Catalog Number: LS-B6212-50
• Alternative Catalog Number: LS-B6212-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: CHEK2 (AAH04207, 1 aa ~ 544 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425

Product Description

CHK2 antibody LS-B6212 is an unconjugated mouse monoclonal antibody to human CHK2 (CHEK2). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.48 mg/ml
• Clone Designation: [4B7]
• NCBI: 11200
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IF (10 µg/ml), IHC, IHC-P (3 - 5 µg/ml), WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Images

Western blot of CHEK2 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CHEK2 is approximately 3 ng/ml as a capture antibody.