IHC-plus(TM) CYB5A / Cytochrome b5 Antibody (clone 1A8) LS-B6526, Clone: [1A8], Unconjugated, Monoclonal

Product Overview

• Article Name: IHC-plus(TM) CYB5A / Cytochrome b5 Antibody (clone 1A8) LS-B6526, Clone: [1A8], Unconjugated, Monoclonal
• Biozol Catalog Number: LS-B6526-50
• Supplier Catalog Number: LS-B6526-50
• Alternative Catalog Number: LS-B6526-50
• Manufacturer: LifeSpan Biosciences
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: CYB5A (AAH15182, 1 aa ~ 135 aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Alternative Names: CYB5A, CYB5, Cytochrome b5 (microsomal), MCB5, Type 1 cyt-b5, Cytochrome b-5, Cytochrome b5

Product Description

Cytochrome b5 antibody LS-B6526 is an unconjugated mouse monoclonal antibody to human Cytochrome b5 (CYB5A). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.49 mg/ml
• Clone Designation: [1A8]
• NCBI: 1528
• Buffer: PBS, pH 7.4
• Purity: Purified from ascites by Protein A
• Form: PBS, pH 7.4

Application Notes

• Application Dilute: ELISA, IHC, IHC-P (5 µg/ml), IP, WB (1:500 - 1:1000)
• Application Notes: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein. For IP 40 ug of antibody per 100 ul of protein A magnetic beads at 50 ug/ml.

Images

Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.

Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.

Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture