Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in othe
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 10% SDS PAGE PROC / Protein C an
IHC of paraffin-embedded Breast ca, using Protein C antibody at 1:250 dilution.
Immunofluorescence of methanol-fixed HeLa, using Protein C antibody at 1:500 dilution.
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