Polyclonal Rabbit anti-Human GRAP Antibody (aa155-217, IHC, WB) LS-C185634

Product Overview

• Article Name: Polyclonal Rabbit anti-Human GRAP Antibody (aa155-217, IHC, WB) LS-C185634
• Biozol Catalog Number: LS-C185634-100
• Supplier Catalog Number: LS-C185634-100
• Alternative Catalog Number: LS-C185634-100
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRAP. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: GRAP, GRB2-related adaptor protein, GRB2-related adapter protein

Product Description

GRAP antibody LS-C185634 is an unconjugated rabbit polyclonal antibody to GRAP (aa155-217) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 10750
• Buffer: 1X PBS, pH 7, 0.01% Thimerosal, 40% Glycerol
• Purity: Immunoaffinity purified
• Form: 1X PBS, pH 7, 0.01% Thimerosal, 40% Glycerol

Application Notes

• Application Dilute: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)
• Application Notes: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Images

Sample (30 ug of whole cell lysate). A: A549 12% SDS PAGE. GRAP antibody diluted at 1:1000.

IHC of paraffin-embedded U373 xenograft using GRAP antibody at 1:100 dilution.

Immunofluorescence of methanol-fixed A549 using GRAP antibody at 1:500 dilution.