Polyclonal Rabbit anti-Human COL3A1 / Collagen III Antibody (aa1247-1389, IHC, WB) LS-C186224

Product Overview

• Article Name: Polyclonal Rabbit anti-Human COL3A1 / Collagen III Antibody (aa1247-1389, IHC, WB) LS-C186224
• Biozol Catalog Number: LS-C186224-100
• Supplier Catalog Number: LS-C186224-100
• Alternative Catalog Number: LS-C186224-100
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: COL3A1, Alpha1 (III) collagen, Collagen alpha 1(iii), Collagen alpha-1(III) chain, Collagen, type III, alpha 1, Collagen, fetal, EDS4A

Product Description

Collagen III antibody LS-C186224 is an unconjugated rabbit polyclonal antibody to human Collagen III (COL3A1) (aa1247-1389). Validated for ICC, IHC and WB.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.67 mg/ml
• NCBI: 1281
• Buffer: 1X PBS, pH 7, 0.025% ProClin(TM) 300, 20% Glycerol
• Purity: Immunoaffinity purified
• Form: 1X PBS, pH 7, 0.025% ProClin(TM) 300, 20% Glycerol

Application Notes

• Application Dilute: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)
• Application Notes: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Images

Sample (30 ug of whole cell lysate). A: Hela. 5% SDS PAGE. COL3A1 antibody d

IHC of paraffin-embedded NCIN87 xenograft using Collagen III alpha1 antibody at 1:500 dilution.

Immunofluorescence of methanol-fixed HeLa using COL3A1 antibody at 1:50 dilution.