Alkaline Phosphatase antibody LS-C209255 is a biotin-conjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Purity:
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form:
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Application Dilute:
ELISA (1:1000 - 1:10000), WB (1:500 - 1:2000)
Application Notes:
This product has been assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:20000 of the reconstitutio
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