Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form:
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Application Dilute:
Peptide-ELISA (1:8000), WB (0.3 - 1 µg/ml)
Application Notes:
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 40-45kDa band observed in nuclear cell lysates of NIH3T3 (calculated MW of 42.8kDa according to Mouse NP_570930.1). Recommended concentration: 0.3-1 ug/ml.
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