Immunoblotting: use at a dilution of 1:20,000-1:400,000. Immunohistochemistry: use at a dilution of 1:500-1:5,000. Immunocytochemistry: use at a dilution of 1:200-1:1,000. ELISA: use at a dilution of 1:20,000-1:400,000. These are recommended dilutions. E
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